Ku adeegsiga tignoolajiyada qaboojinta heerkulbeegga ee aaladaha PCR

Ku adeegsiga tignoolajiyada qaboojinta heerkulbeegga ee aaladaha PCR

Codsiga tignoolajiyada qaboojinta heerkulbeegga ee aaladaha PCR waxay inta badan ku jirtaa xakamaynta heerkulka. Faa'iido udub dhexaadkeedu waa awooda xakamaynta heerkulka degdega ah oo saxda ah, taas oo hubinaysa heerka guusha ee tijaabooyinka xoojinta DNA.

Xaaladaha codsiga muhiimka ah

1. Xakamaynta heerkulka saxda ah

Qalabka PCR wuxuu u baahan yahay inuu wareego iyada oo loo marayo saddex marxaladood: Denaturation heerkulka sare (90-95 ℃), heerkulka hooseeya (55-65 ℃), iyo heerkulka ugu fiican (70-75 ℃). Hababka qaboojinta dhaqameed way adagtahay in la buuxiyo saxnaanta shuruudaha ± 0.1℃. Qaboojinta kuleyliyaha, tignoolajiyada qaboojinta peltier waxay ku guulaysataa nidaaminta heerkulka heerka millisecond iyada oo loo marayo saamaynta Peltier, iyada oo laga fogaanayo guul-darrida kordhinta ee ay keento farqiga heerkulka 2℃.

2. Qaboojin degdeg ah iyo kuleyl

Qaybaha qaboojinta kuleyliyaha, qalabka korantada, qalabka peltier, modules peltier wuxuu gaari karaa heerka qaboojinta 3 ilaa 5 darajo Celsius ilbiriqsikii, si weyn u gaabinaya wareegga tijaabada marka la barbar dhigo 2 darajo Celsius halkii labaad ee kombaresarada dhaqameed. Tusaale ahaan, qalabka PCR ee 96-fiican wuxuu qaataa tignoolajiyada xakamaynta heerkulka aagga si loo hubiyo heerkul joogto ah dhammaan meelaha wanaagsan lagana fogaado kala duwanaanshaha heerkulka 2℃ ee ay keento saamaynta cirifka.

3. Kor u qaadida isku halaynta qalabka

Qaybaha qaboojinta heerkulbeegyada, modules peltier, lements peltier, modules TEC ee Beijing Huimao Cooling Equipment Co., Ltd. waxay noqdeen qaybaha ugu muhiimsan ee xakamaynta heerkulka aaladaha PCR sababtoo ah isku halaynta sare. Xajmiga yar iyo sifooyinka aan sanqadh lahayn ayaa ka dhigaya mid ku habboon shuruudaha saxda ah ee qalabka caafimaadka.

Kiisaska codsiga caadiga ah

Qalabka PCR-ga ah ee 96-wanaagsan ee fluorescence-ga ah: Isku-dhafan module qaboojin heerkul-koronto, module TEC, aaladda peltier, modules peltier waxay awood u siinaysaa xakamaynta heerkulka saxda ah ee shaybaarrada-soo-saarka sare waxaana si ballaaran loo adeegsadaa goobaha sida falanqaynta muujinta hiddaha iyo ogaanshaha cudurka.

Talaagadaha caafimaadka ee la qaadi karo: qaboojinta heerkulbeegyada, qaboojinta qaboojinta qaboojinta ee la qaadi karo ee loo isticmaalo in lagu kaydiyo alaabta sida tallaalada iyo daawooyinka u baahan jawi kuleyl hooseeya, hubinta xasiloonida heerkulka inta lagu jiro gaadiidka.

Qalabka daaweynta laser:

Qaybaha qaboojinta kuleyliyaha, canaasiirta peltier, modules-kuleylku waxay qaboojiyaan hawada sare ee laysarka si loo yareeyo khatarta gubashada maqaarka oo kor loogu qaado badbaadada daawaynta.

TEC1-39109T200

Heerkulka dhinaca kulul waa 30 C,

imax: 9A

Umax: 46V

Qmax: 246.3W

ACR: 4± 0.1Ω (Ta= 23 C)

Delta T ugu badnaan: 67-69C

Cabbirka: 55x55x3.5-3.6mm

TES1-15809T200

Heerkulka dhinaca kulul: 30 C,

Imax: 9.2A

Umax: 18.6V

Qmax: 99.5 W

Delta T ugu badnaan: 67C

ACR: 1.7 ± 15% Ω (1.53 ilaa 1.87 Ohm)

Cabbirka: 77×16.8×2.8mm

Silig: 18 AWG silikoon silig ama siman Sn-ku dahaadhay dusha sare, iska caabin heerkul sarreeya 200 ℃